Thanks for your feedbacks. I was not using hardware RAID on DSS Lite or iSCSI R3. On iSCSI R3 I use software RAID 5 and on DSS Lite I'm not sure what I did configure. But I know I used default Block IO with DSS Lite and got good performance while testing with one VM and IOMeter. Testing few virtual disks with IOMeter I never did. Now I cannot play with my DSS Lite because the activation is gone and I dont want to start the activation procedure with putting the maschine in front of our firewall...
When I use Block IO with iSCSI R3, I got no message box telling me that File IO is suggested with VMWare.
I will use File IO with my iSCSI R3 and give up playing with Block IO.
What I wish is more support from Open-E giving us information what are the best settings using their products with VMWare. I was reading Block IO is 30% faster than File IO. So I moved all our VMs to another storage, made the update, recreated a volume with Block IO just to realize the performance is pretty poor and File IO is recommended for use with VMWare.