Your iSCSI Enterprise "1.72" will not replicate to the iSCSI-R3 Enterprise. Only iSCSI-R3 Enterprise will replicate to another iSCSI-R3 or DSS.
For DSS and iSCSI-R3 you can enable replication for an existing volume without losing data as well as removing and adding it again just don't delete the volume.
If the target is removed then connection to target will no longer be available and will not reset the other connections to other luns or or targets, also it is recommended to allow the replication to complete first before writing to the target as this will speed up the replication (best to add a dedicated NIC). Once the replication is completed only enable the target for the source not the destination target.
We don't have a way to upgrade the iSCSI Enterprise to iSCSI-R3 Enterprise as they are different logical volume managers and the USB build is larger the the IDE module.
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